Collection of islets of Langerhans using an equilibrium method.

نویسندگان

  • Duk-Su Koh
  • Mark Moody
  • Junghyo Jo
چکیده

Here we present a convenient method for easy hand selection of enzymatically isolated small tissues such as islets of Langerhans. Islets are continuously collected in a micropipette tip connected to a peristaltic pump. After entering the conical micropipette tip, the islets are quickly dragged up by solution flow, but this movement subsequently decreases as the flow rate decreases. Thus, the islets are trapped at a specific height where downward gravitation balances upward buoyancy and the drag provided by solution flow. Our device allows more efficient isolation of islets compared to conventional manual collection methods.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

اثر متفورمین بر روی حجم جزایر لانگرهانس درپانکراس موش سوری

Background and Objective: Metformin is a widely used medicine for treatment of type 2 diabetes. In this study, the effect of various doses of metformin on the mouse islets of langerhans volume was investigated. Materials and methods: Twenty four C57BL/6 adult male mice weighting 30±5 gr were randomly divided into 4 groups. Normal saline was given to the control group (group 4) and the experimen...

متن کامل

MONOPHASIC STATE OF INSULIN SECRETION IN ISOLATED RAT ISLETS OF LANGERHANS BY K+, Ca2+ AND GLUCOSE

Isolation of rat islets of Langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of Hanks' solution into the common bile duct. The identification of the islets was done by techniques including the use of a stereomicroscope against a black background. Vital staining was done by an injection of neutral red solution through t...

متن کامل

Improvement of the functionality of pancreatic Langerhans islets via reduction of bacterial contamination and apoptosis using phenolic compounds

Objective(s): During type-1 diabetes treating by pancreatic islet transplantation, increasing oxidative stress and microbial contaminations are the main reasons of transplantation failure. In this study, we evaluated anti-apoptotic, antioxidant and antimicrobial potentials of phenolic compounds called ellagic acid (EA) and silybin on rat pancreatic islets. Materials and Methods: By doing MTT as...

متن کامل

Normal Insulin Secretion from Immune-Protected Islets of Langerhans by PEGylation and Encapsulation in the Alginate-Chitosan-PEG

Background: Pancreatic islet transplantation is one of the most promising strategies for treating patients with type I diabetes mellitus.Objective: We aimed to assess the immunoisolation properties of the multilayer encapsulated islets using alginate-chitosan-PEG for immunoprotection and insulin secretion from the encapsulated islets induced under differe...

متن کامل

Effect of sex on histomorphometric properties of Langerhans islets in native chickens

The aim of the present study was to investigate the effect of gender on the distribution of pancreatic islets in native chicken. Ten adult male and ten adult female Isfahan native chickens were used in this experiment. Results showed a distinct sexual dimorphism in the native chicken pancreas which depends upon the various fractions of the pancreatic lobes, which were occupied by alpha, beta an...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • BioTechniques

دوره 55 1  شماره 

صفحات  -

تاریخ انتشار 2013